| Strength: | |
|---|---|
| Quantity: | |
SJB07
10μg, 100μg
Description:
Recombinant human IL-6 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids. Recombinant human IL-6 is different from natural IL-6 with only one proline replaced by serine at the N-terminal, but no difference in activity between the two in vivo and in vitro.
Source: E. coli.
Molecular Mass: Approximately 20.9kD.
Purity: ≥97%.
Endotoxin Level: <1EU/µg, determined by the LAL method.
Biological Activity:
Measured in a cell proliferation assay using B9-11, the specific activity shall be not less than 1×107IU/mg.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4.
Reconstitution:
It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.
Storage:
Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
For Laboratory Use Only.
Description:
Recombinant human IL-6 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids. Recombinant human IL-6 is different from natural IL-6 with only one proline replaced by serine at the N-terminal, but no difference in activity between the two in vivo and in vitro.
Source: E. coli.
Molecular Mass: Approximately 20.9kD.
Purity: ≥97%.
Endotoxin Level: <1EU/µg, determined by the LAL method.
Biological Activity:
Measured in a cell proliferation assay using B9-11, the specific activity shall be not less than 1×107IU/mg.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4.
Reconstitution:
It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.
Storage:
Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
For Laboratory Use Only.
