| Quantity: | |
|---|---|
SJB06
25μg
Description:
Recombinant human IL-18 produced in yeast is a polypeptide chain containing 157 amino acids, with 4 Cys, but no disulfide bond.
Source: Yeast.
Molecular Mass: Approximately 18.2kD.
Purity: ≥97%.
Endotoxin Level: <1EU/µg, determined by the LAL method.
Biological Activity:
Induction of IFN-γ by KG-1 cell in response to the recombinant human IL-18 is measured using human IFN-γ ELISA.
The activity should be as follow:
IL-18 concentration (ng/ml) IFN-γ induction (IU/ml)
80 ≥15
40 ≥10
IFN-γ producing activity of the sample cells can be varied depends on cell conditions. Optimal concentration for each application should be determined by each laboratory.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS containing 0.2% HSA, pH7.4
Reconstitution:
It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.
Storage:
Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
For Laboratory Use Only.
Description:
Recombinant human IL-18 produced in yeast is a polypeptide chain containing 157 amino acids, with 4 Cys, but no disulfide bond.
Source: Yeast.
Molecular Mass: Approximately 18.2kD.
Purity: ≥97%.
Endotoxin Level: <1EU/µg, determined by the LAL method.
Biological Activity:
Induction of IFN-γ by KG-1 cell in response to the recombinant human IL-18 is measured using human IFN-γ ELISA.
The activity should be as follow:
IL-18 concentration (ng/ml) IFN-γ induction (IU/ml)
80 ≥15
40 ≥10
IFN-γ producing activity of the sample cells can be varied depends on cell conditions. Optimal concentration for each application should be determined by each laboratory.
Formulation:
Lyophilized from a 0.2μm filtered solution in PBS containing 0.2% HSA, pH7.4
Reconstitution:
It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.
Storage:
Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.
Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.
Avoid repeated freeze-thaw cycles.
For Laboratory Use Only.
